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Thorlabs microscope cube assembly nikon te2000
Microscope Cube Assembly Nikon Te2000, supplied by Thorlabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+cube+assembly+nikon+te2000/microscope+cube+assembly+nikon+te2000/pmc07920322-57-56-57
Average 90 stars, based on 1 article reviews
microscope cube assembly nikon te2000 - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Super-resolution Microscopy at Cryogenic Temperatures Using Solid Immersion Lenses
Article Snippet: super SIL assembly Hyper Hemispheric Ball lens, Cubic Zirconia, 0.89 mm diameter x 0.73 mm thickness, λ\4 flatness (Knight Optical, LBB2018-C) Platinum Foil (Goodfellow, catalog number: PT000264 876-861-73) Cryo-compatible adhesive (Loctite Stycast, 2850 FT) Preparation of bacterial and fiducial solutions Flat cap centrifuge tubes, 50 ml (Fisherbrand, catalog number: 05-539-13) Eppendorf tubes, 2 ml (Eppendorf, catalog number: 0030 123.344) Tetraspeck 0.1 μm blue/green/orange/dark red beads (Molecular probes, Thermo Fisher Scientific, catalog number: T7279) Agar plate(s) with colonies of E.Coli expressing plasmid of interest LB medium granular ( e.g. , Melford, L24400-5000.0) 1 M HCl solution in water for pH adjustment (made up from HCl 37% ACS grade, 320331, Sigma-Aldrich) Selection antibiotics stock solutions in water ( e.g. , Kanamycin 25 mg/ml from powder-Sigma-Aldrich, K1377; Carbenicillin 100 mg/ml ready-made solution, Sigma-Aldrich, C1613) IPTG 1 M stock solution in water (made up from powder e.g. , Fluorochem, M02726) PBS 1x pH 7.4 (Gibco, catalog number: 10010-015) Ultrapure deionized water (MilliQ or equivalent) LB medium (see Recipes) Cleaning of super SIL assemblies Clean glass slides ( e.g. , Fischer Scientific 16 x 38 mm microscope slides 244879) Filter paper ( e.g. , qualitative filter paper grade A, SLS 2036) Clean glass scintillation vials ( e.g. , Thermo Chromacol 20 ml vials 20-EPSVCA) super SIL assemblies (produced as described in Procedure A), cubic zirconia crystal NA 2.17 Sulphuric acid ≥ 95% pure ( e.g. , Fischer Scientific S/9231/PB15) 30% Hydrogen Peroxide with inhibitor, ACS grade ( e.g. , Sigma-Aldrich, catalog number: 216763) At least 100 ml of MilliQ (or equivalent) water Piranha Solution (see Recipes) Plunge-freezing Ethane gas bottle Cleaned and glow discharged super SILs (prepared as described in Procedure D) Bacterial suspension (prepared as described in Procedure B) Fiducial suspension (prepared as described in Procedure C) Liquid nitrogen (at least a full 4 L dewar) 70% ethanol MilliQ water .. list-behavior=enumerated prefix-word= mark-type=upper-alpha max-label-size=0 super SIL assembly and microscope list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Ceramic hot plate (VWR, catalog number: 444-0624) Coordinate measuring machine (OGP, SmartScope ZIP 250) Microscope stand list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Cerna Microscope Body with Epi-Illumination Arm (Thorlabs, catalog number: CEA1350) Nikon D-FL Epi-Fluorescence Illuminator (Thorlabs, catalog number: CSE1000) Microscope cube assembly for Nikon TE2000 (Thorlabs, catalog number: TLV-TE2000) Single objective arm (Thorlabs, catalog number: CSA1100) Objective focusing module (Thorlabs, catalog number: ZFM2020) 3-axis controller and knob box for 1" Cerna stages (Thorlabs, catalog number: MCM3001) TIRF module comprising of X/Y translator and right-angle mirror mount (Thorlabs, ST1XY-D and KCB1/M) Translation stage (Prior, H117P2IX/G) Translation stage controller (Prior, ProScan III H31XYZEF) Image splitting module (Cairn, Twincam) Optics, sample stage, and cameras The following components are used in the microscope schematic illustrated in . .. In principle, most popular commercial microscope stands could be modified to create a similar configuration since various adapter plates for the cryo-stage are available from Linkam Scientific Ltd and the Cairn Twincam can be purchased with c-mount threads on the input and output ports to suit most types of scientific camera. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 1. caption a7 caption a8 Microscope schematic detailing optics layout Note: Check the emission spectra of your fluorophore or dye of choice at cryogenic temperature and select dichroic and emission filters to suit.



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Thorlabs microscope cube assembly nikon te2000
Microscope Cube Assembly Nikon Te2000, supplied by Thorlabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+cube+assembly+nikon+te2000/microscope+cube+assembly+nikon+te2000/pmc07920322-57-56-57
Average 90 stars, based on 1 article reviews
microscope cube assembly nikon te2000 - by Bioz Stars, 2026-10
90/100 stars
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